The Occurrence of Plant Pathogenic Streptomyces spp. in Potato-growing Regions in Central Europe
Identifieur interne : 001D86 ( Main/Exploration ); précédent : 001D85; suivant : 001D87The Occurrence of Plant Pathogenic Streptomyces spp. in Potato-growing Regions in Central Europe
Auteurs : Iveta Pánková [République tchèque] ; Vladimíra Sedláková [République tchèque] ; Petr Sedlák [République tchèque] ; Václav Krejzar [République tchèque]Source :
- American Journal of Potato Research [ 1099-209X ] ; 2012-06-01.
English descriptors
Abstract
Abstract: Thirty-three isolates of Streptomyces from lesions on potato tubers and nine Streptomyces type strains were identified by fatty acid analysis and PCR and their pathogenicity was tested. The results were analysed using the unweighted pair group method algorithm. Pathogenicity and the genetic constitution of the pathogenicity island (PAI) were found to be correlated, and the results of fatty acid analysis and PCR correlated strongly for 34 % of the isolates. Altogether, 82 % of the isolates closely resembled the Streptomyces scabies type strain DSMZ 41658T included in this study. Isolates from regions with different meteorological and soil conditions and with different levels of pathogenicity formed clusters when grouped on the basis of fatty acid composition and DNA polymorphism. The most virulent isolates, namely Bee, Judita, and Madeleine, were identified as Streptomyces scabies. However, results of FAME, RAPD analysis, and pathogenicity tests differed significantly in 18 % of the isolates, and this cluster should be examined in greater detail in the future.
Resumen: Mediante un análisis de ácidos grasos y PCR, se identificaron 33 aislamientos y nueve variantes de Streptomyces de lesiones en tubérculos de papa y se probó su patogenicidad. Se analizaron los resultados mediante un método de algoritmos de grupos por pares no ponderados. Encontramos que la patogenicidad y la constitución de la isla de dicha patogenicidad (PAI) estaban correlacionados, y los resultados de los análisis de los ácidos grasos y la PCR se correlacionaron fuertemente para 34 % de los aislamientos. En conjunto, 82 % de los aislamientos tuvieron un parecido cercano a Streptomyces scabies variante tipo DSMZ 41658 T incluido en este estudio. Los aislamientos de regiones con diferentes condiciones meteorológicas y de suelo y con diferentes niveles de patogenicidad formaron congregados cuando se agruparon con base a la composición de los ácidos grasos y del polimorfismo de ADN. Los aislamientos mas virulentos, como Bee, Judita, y Madeleine, se identificaron como Streptomyces scabies. No obstante, los resultados de FAME, RAPD, y las pruebas de patogenicidad difirieron significativamente en 18 % de los aislamientos, y este agrupamiento debería examinarse en mayor detalle a futuro.
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DOI: 10.1007/s12230-012-9245-4
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J. Pot Res</title><idno type="ISSN">1099-209X</idno><idno type="eISSN">1874-9380</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>New York</pubPlace><date type="published" when="2012-06-01">2012-06-01</date><biblScope unit="volume">89</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="207">207</biblScope><biblScope unit="page" to="215">215</biblScope></imprint><idno type="ISSN">1099-209X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1099-209X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Common scab disease</term><term>Fatty acid composition</term><term>Pathogenicity test</term><term>Polymerase chain reaction</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Thirty-three isolates of Streptomyces from lesions on potato tubers and nine Streptomyces type strains were identified by fatty acid analysis and PCR and their pathogenicity was tested. The results were analysed using the unweighted pair group method algorithm. Pathogenicity and the genetic constitution of the pathogenicity island (PAI) were found to be correlated, and the results of fatty acid analysis and PCR correlated strongly for 34 % of the isolates. Altogether, 82 % of the isolates closely resembled the Streptomyces scabies type strain DSMZ 41658T included in this study. Isolates from regions with different meteorological and soil conditions and with different levels of pathogenicity formed clusters when grouped on the basis of fatty acid composition and DNA polymorphism. The most virulent isolates, namely Bee, Judita, and Madeleine, were identified as Streptomyces scabies. However, results of FAME, RAPD analysis, and pathogenicity tests differed significantly in 18 % of the isolates, and this cluster should be examined in greater detail in the future.</div><div type="abstract" xml:lang="es">Resumen: Mediante un análisis de ácidos grasos y PCR, se identificaron 33 aislamientos y nueve variantes de Streptomyces de lesiones en tubérculos de papa y se probó su patogenicidad. Se analizaron los resultados mediante un método de algoritmos de grupos por pares no ponderados. Encontramos que la patogenicidad y la constitución de la isla de dicha patogenicidad (PAI) estaban correlacionados, y los resultados de los análisis de los ácidos grasos y la PCR se correlacionaron fuertemente para 34 % de los aislamientos. En conjunto, 82 % de los aislamientos tuvieron un parecido cercano a Streptomyces scabies variante tipo DSMZ 41658 T incluido en este estudio. Los aislamientos de regiones con diferentes condiciones meteorológicas y de suelo y con diferentes niveles de patogenicidad formaron congregados cuando se agruparon con base a la composición de los ácidos grasos y del polimorfismo de ADN. Los aislamientos mas virulentos, como Bee, Judita, y Madeleine, se identificaron como Streptomyces scabies. No obstante, los resultados de FAME, RAPD, y las pruebas de patogenicidad difirieron significativamente en 18 % de los aislamientos, y este agrupamiento debería examinarse en mayor detalle a futuro.</div></front></TEI><affiliations><list><country><li>République tchèque</li></country><region><li>Bohême centrale</li></region><settlement><li>Prague</li></settlement></list><tree><country name="République tchèque"><region name="Bohême centrale"><name sortKey="Pankova, Iveta" sort="Pankova, Iveta" uniqKey="Pankova I" first="Iveta" last="Pánková">Iveta Pánková</name></region><name sortKey="Krejzar, Vaclav" sort="Krejzar, Vaclav" uniqKey="Krejzar V" first="Václav" last="Krejzar">Václav Krejzar</name><name sortKey="Pankova, Iveta" sort="Pankova, Iveta" uniqKey="Pankova I" first="Iveta" last="Pánková">Iveta Pánková</name><name sortKey="Sedlak, Petr" sort="Sedlak, Petr" uniqKey="Sedlak P" first="Petr" last="Sedlák">Petr Sedlák</name><name sortKey="Sedlakova, Vladimira" sort="Sedlakova, Vladimira" uniqKey="Sedlakova V" first="Vladimíra" last="Sedláková">Vladimíra Sedláková</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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